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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site

Fig. 1

Blocking IL-10 at the time of immunisation does not increase the survival time of TC-1 tumour bearing mice a Groups of five C57BL/6 mice were primed and boosted on day 0 and day 7 respectively with 40 μg of Ex peptides (each 10 μg), 15 mg of MPLA and 250 μg of anti-IL10 receptor antibodies or IgG Isotype control antibody subcutaneously. Seven days after final immunisation, spleens from immunized mice were collected, single spleen cells isolated and cultured in the presence of an MHC I restricted HPV16 E7 specific peptide RAHYNIVTF. ELISPOT assay for IFNγ was performed as described in Materials and Methods. b, c Groups of five C57BL/6 mice were immunized with 40 μg of Ex peptide, 15 mg of MPLA and 250 μg of anti-IL10 receptor antibodies or IgG isotype control antibody subcutaneously or left unimmunized on day zero and seven. Seven days after immunization, 2 × 105 of TC-1 tumour cells were inoculated subcutaneously in the flank of immunized or unimmunised mice, and tumour size and survival of mice were monitored as described in Materials and Methods. (D, E) Groups of eight C57BL/6 mice were inoculated subcutaneously in the flank with 2 × 105 of TC-1 tumour cells. 5 days later, mice were immunized with 40 mg of Ex peptide, 15 mg of MPLA and 250 μg of anti-IL10 receptor antibodies or IgG isotype control antibody subcutaneously or left unimmunized twice 7 days apart. Survival of mice was monitored as described in Materials and Methods. The results represent at least one of two independent experiments.

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