Fig. 3

Costunolide changed the ΔΨm and induced mitochondria-mediated apoptosis in gastric adenocarcinoma cells. a ΔΨm of BGC-823 cells treated by costunolide with different concentration (0, 5, 10, 15, 20 and 25 μM) for 24 h and stained by JC-1 is detected by flow cytometry. b Statistical data of ΔΨm change ratio. c Apoptosis related proteins (Bax, Bcl-2, pro-caspase 9, cleaved caspase 9, pro-caspase 7, cleaved caspase 7, pro-caspase 3, cleaved caspase 3, PARP and cleaved PARP, β-actin is internal reference protein) of BGC-823 cells treated by costunolide with different concentration (0, 5, 10, 15, 20 and 25 μM) for 24 h is detected by WB. d Apoptosis related genes (puma, Bak1, Bax and Bcl-2, GAPDH is internal reference gene) expression of BGC-823 cells treated by costunolide with different concentration (0, 5, 10, 15, 20 and 25 μM) for 24 h is detected by Q-PCR. Compared to control group, *P < 0.05, **P < 0.01