Fig. 2

Costunolide induced the apoptosis of gastric adenocarcinoma BGC-823 cells. a Hoechst33258 staining of BGC-823 cells treated by costunolide with different concentration (0, 5, 10, 15, 20 and 25 μM) for 24 h are photographed by fluorescence microscope (magnification: 200×, bar in the pictures represents 20 μm). b Statistical data of Hoechst33258 positive cells. c Apoptosis of BGC-823 cells treated by costunolide with different concentration (0, 5, 10, 15, 20 and 25 μM) for 24 h and stained by Annexin V-FITC and PI is detected by flow cytometry. d Statistical data of BGC-823 cell apoptosis. Compared to control group, *P < 0.05, **P < 0.01