Table 4 The stability of cucurbitacin B at different storage conditions
From: Pharmacokinetics of cucurbitacin B from Trichosanthes cucumerina L. in rats
Conditions | QC concentration (μg/L) | Measured values (μg/L) | SD | %CV | %RE | %Recovery |
|---|---|---|---|---|---|---|
Room temperature (12 h) | 0.5 | 0.43 | 0.02 | 4.90 | 14.20 | 81.34 |
10 | 8.20 | 0.19 | 2.34 | 18.01 | 83.32 | |
15 | 11.80 | 0.15 | 1.25 | 21.35 | 86.16 | |
Storage at −20 °C (3 months) | 0.5 | 0.48 | 0.03 | 5.66 | 4.60 | 90.54 |
10 | 9.81 | 0.65 | 6.58 | 1.86 | 99.72 | |
15 | 13.46 | 0.42 | 3.10 | 10.29 | 97.93 | |
Three freeze-thaw cycles | 0.5 | 0.49 | 0.03 | 6.39 | 3.00 | 92.01 |
10 | 9.79 | 0.22 | 2.21 | 2.13 | 99.50 | |
15 | 13.68 | 0.46 | 3.34 | 8.81 | 99.54 | |
Autosampler (10 °C) | 0.5 | 0.47 | 0.03 | 7.25 | 6.20 | 89.05 |
10 | 10.78 | 0.76 | 7.01 | −7.78 | 109.57 | |
15 | 13.84 | 0.39 | 2.82 | 7.71 | 100.75 |