Table 5 Effect of RHTR on biochemical and hematological parameters of BALB/c mice in the acute toxicity study

 Glucose (mg/dL)

118.7 ± 13.8

113.7 ± 17.5

138.7 ± 17.9

130.2 ± 6.9

127.7 ± 15.4

141.5 ± 10.4

63–176 (89)

 Triglycerides (mg/dL)

70.13 ± 11.3

74.6 ± 7.4

81.4 ± 8.6

121.8 ± 17.2

112.75 ± 19.8

98.5 ± 7.3

55–115 (85)

 Cholesterol (mg/dL)

76.3 ± 8.5

77.5 ± 14.4

75 ± 8.2

61 ± 12.7

55 ± 5.8

65 ± 2.8

26–82 (64)

 Protein (g/dL)

5.4 ± 0.5

5.5 ± 0.6

5.4 ± 0.25

5.7 ± 0.63

5.2 ± 0.5

5.8 ± 0.82

4–8.6 (6.2)

 AST (TGO) (U/L)

205 ± 45.3 *

152.5 ± 8.7

127.5 ± 19.4

132 ± 19.7

165 ± 45.5

161.8 ± 47.5

55–251 (139)

 ALT (TGP) (U/L)

55.9 ± 8.8

78.7 ± 0.3 *

52.9 ± 6.2

64.4 ± 1.4

74.7 ± 16.1

70.7 ± 5.8

17–77 (47)

 LDH (U/L)

1234 ± 221

954 ± 281

1330 ± 274

1122 ± 201

980 ± 286

997.3 ± 70.3

149–215 (182)

 Creatinine (mg/dL)

0.62 ± 0.2

0.5 ± 0.01

0.5 ± 0.01

0.5 ± 0.01

0.5 ± 0.01

0.46 ± 0.09

0.2–0.9 (0.5)

 Urea (mg/dL)

60 ± 5.8

56.2 ± 4.8

53.7 ± 6.3

65 ± 4.1

57.5 ± 6.5

56.7 ± 7.2

46.9–73 (60.1)

 Hemoglobin (g/dL)

15.16 ± 1.09

13.5 ± 0.36

14.76 ± 0.90

16 ± 1.05

13.23 ± 1.8

14.13 ± 0.20

10–17 (13.1)

 Hematocrit (%)

47.2 ± 4.07

39.33 ± 3.40

43.93 ± 2.83

49.5 ± 3.99

38.8 ± 5.63

43.7 ± 0.55

39–49 (40.4)

 Erythrocytes (X10⁶/mm³)

9.69 ± 1.16

7.34 ± 0.95

9.27 ± 0.97

9.92 ± 0.85

7.91 ± 1.13

8.84 ± 0.43

8.3

 MCHC (%)

32.06 ± 0.66

36.36 ± 4.56

33.63 ± 1.04

32.3 ± 0.52

36.26 ± 2.57

32.3 ± 0.72

32.3

 MCH (pg)

15.66 ± 0.8

14.63 ± 3.68

15.96 ± 0.96

16.1 ± 0.34

14.3 ± 2.4

15.96 ± 0.86

15.9

 MCV (μL)

48.9 ± 1.6

47.26 ± 11.49

47.5 ± 1.83

49.9 ± 0.3

48.9 ± 1.95

49.5 ± 1.83

49.1

 Leukocytes (×  100/mm³)

5000 ± 953.9

4233.3 ± 1404.7

5666.66 ± 808.29

4466.66 ± 929.15

4666.6 ± 1242.3

5033.33 ± 1227.8

5–12 (6.33)

 RDW-CV

15 ± 1.17

18.7 ± 1.56

17.96 ± 1.11

13.73 ± 0.57 *

16.33 ± 0.97

16.16 ± 1.20

 RDW-SD

24.26 ± 3.18 *

32.63 ± 18.9

33.2 ± 5.32

22.8 ± 0.51*

26.13 ± 1.5

25.73 ± 1.38

 Platelets (/μL)

437,333 ± 94,214

558,666.6 ± 230,048.5

627,000 ± 95,859.2

368,000 ± 46,130

425,333.3 ± 325,423

572,666 ± 158,190

116 × 10³

 MPV (fL)

7.6 ± 0.2

7.5 ± 2.4

8.16 ± 0.87

7.56 ± 0.15

9.43 ± 0.49 *

7.6 ± 0.3

 PDW (%)

14 ± 0.1

14.6 ± 4.5

14.96 ± 2.32

14.06 ± 0.15

13.03 ± 2.8

14 ± 0.26

 PCT (%)

0.33 ± 0.07 *

0.27 ± 0.05 *

0.51 ± 0.12

0.277 ± 0.03 *

0.585 ± 0.12

0.432 ± 0.10

 Lymphocytes (%)

67.33 ± 10.06 *

74 ± 7.21

82 ± 2

65 ± 13.4

63.3 ± 4.16

68 ± 20.7

35–90

 Monocytes (%)

1.33 ± 1.15

0

0.33 ± 0.57

0.33 ± 0.57

0

0

0–3

 Eosinophils (%)

2

0

0

0

0

0

0–7

 Basophils (%)

0

0

0

0

0

0

0–1

 Segmented neutrophils (%)

38 ± 9.16 *

25.3 ± 7.5

17.66 ± 2.08

34.66 ± 13.61

16.66 ± 4.16

24.66 ± 27.30

10–40

 Neutrophils band (%)

0

0

0

0

0

0

0

 Immature shape (%)

0

0

0

0

0

0

0