Fig. 3From: Biological and toxicological evaluation of Rhus trilobata Nutt. (Anacardiaceae) used traditionally in mexico against cancerBiological activity of RHTR in normal cell lines. The toxicological effects of AE and FF from RHTR in CHO-K1 (a) and BEAS-2B (b) cells were evaluated using dose-response viability curves for 24 h by the MTT assay. The morphological changes with Hemacolor® rapid staining and induction of apoptosis by TUNEL assay were observed after 24 h of treatment with the IC50 of AE and FF (c). The migration of damaged DNA was determined by single cell gel electrophoresis under alkaline conditions (comet assay) after treatment for 24 h with AE and FF at 600 μg/mL in CHO-K1 cells (d). The production of intracellular ROS in CHO-K1 cells was determined by CM-H2DCFDA assays under the same conditions mentioned above (e). The results show the mean ± S.D. of three biological replicates (n = 3, in triplicates); *, p < 0.05 vs the control group without treatment (1X PBS)Back to article page