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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Biological and toxicological evaluation of Rhus trilobata Nutt. (Anacardiaceae) used traditionally in mexico against cancer

Fig. 3

Biological activity of RHTR in normal cell lines. The toxicological effects of AE and FF from RHTR in CHO-K1 (a) and BEAS-2B (b) cells were evaluated using dose-response viability curves for 24 h by the MTT assay. The morphological changes with Hemacolor® rapid staining and induction of apoptosis by TUNEL assay were observed after 24 h of treatment with the IC50 of AE and FF (c). The migration of damaged DNA was determined by single cell gel electrophoresis under alkaline conditions (comet assay) after treatment for 24 h with AE and FF at 600 μg/mL in CHO-K1 cells (d). The production of intracellular ROS in CHO-K1 cells was determined by CM-H2DCFDA assays under the same conditions mentioned above (e). The results show the mean ± S.D. of three biological replicates (n = 3, in triplicates); *, p < 0.05 vs the control group without treatment (1X PBS)

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