Fig. 2

Biological activity of RHTR in colon cancer cells. The antiproliferative effects of AE and FF from RHTR were evaluated from 24 to 72 h with the IC₅₀ previously determined by the MTT assay (a). The morphological changes and induction of apoptosis were determined after 24 h of treatment with AE (5 μg/mL) and FF (18 μg/mL) by Hemacolor® rapid staining and the TUNEL assay, respectively (b). The DNA content in different phases of the cell cycle during treatments with RHTR was determined at 24 h under the same conditions mentioned above by flow cytometry with PI (c). The results show the mean ± S.D. of three biological replicates (n = 3, in triplicates); *, p < 0.05 vs the control group without treatment (1X PBS)