Table 1 Quantitative HPLC analyses of composition in CG extraction
Peak | ESI-MS RT (min) | UV (nm) | Detected ion (m/z) | Calculated ion (m/z) | Error (ppm) | MS-MS ions (m/z) | Tentative identification | Molecular formula |
|---|---|---|---|---|---|---|---|---|
1 | 3.17 | 208, 254, 352 | 609.1424 | 609.1456 | −5.3 | 179, 300, 301 | Quercetin 3-rutinoside | C27H30O6 |
2 | 3.35 | 208, 265, 344 | 593.1505 | 593.1506 | −0.2 | 179, 284 | Kaempferol-4′-O-rutinoside | C27H30O15 |
3 | 3.45 | 203, 254, 352 | 593.1472 | 593.1506 | −5.7 | 151, 179, 285 | Kaempferol-3-O-rutinoside | C27H30O15 |
4 | 3.52 | 203, 254, 353 | 623.1595 | 623.1612 | −2.7 | 179, 315, 593 | Isorhamnetin-3-O-rutinoside | C28H32O16 |
5 | 3.84 | 219 | 697.3453 | 697.3470 | −2.4 | 389, 535 | Deglucoerycordin | C35H54O14 |
6 | 4.21 | 225 | 547.2565 | 547.2543 | 4.0 | 179, 343, 401, 419 | 15b-hydroxycalotropin | C28H40O10 |
7 | 4.42 | 225 | 535.2933 | 535.2907 | 0.7 | 179, 389 | Frugoside | C29H44O5 |
8 | 5.62 | 225 | 329.2387 | 329.2328 | 5.9 | 171, 206, 211 | Trihydroxy octadecenoic acid | C18H34O5 |