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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Calotropis gigantea extract induces apoptosis through extrinsic/intrinsic pathways and reactive oxygen species generation in A549 and NCI-H1299 non-small cell lung cancer cells

Fig. 5

Effects of CG on extrinsic apoptosis pathway-related factors in A549 and NCI-H1299 cells. mRNA expression of DR5, FADD, Fas, and FasL in A549 (a) and NCI-H1299 (b) cells, as determined by qPCR analysis. The graph was compiled from at least three replicate analyses. Protein expression of the extrinsic pathway factors, pro-caspase-8 and its cleaved form, in A549 (c) and NCI-H1299 (d) cells, as determined by western blotting. The cells were treated with various concentrations of CG for 48 h and compared with untreated cells. The data are presented as the mean ± SEM (n = 3). The data were analyzed using one-way ANOVA with Tukey’s HSD test. *, p < 0.05 and **, p < 0.005. Pro cas-8, pro-caspase-8; Cleav-cas-8, cleaved caspase-8

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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