Fig. 4

Effects of CG on the cell cycle phases in A549 and NCI-H1299 cells. a Protein expression of p53, p27, p21, and GAPDH in A549 cells and b protein expression of p27, p21, and GAPDH in NCI-H1299 cells as determined by western blotting. A549 and NCI-H1299 cells were treated with various concentrations of CG for 48 h and compared with untreated cells. The cell cycle profiles of CG-treated A549 (c) and NCI-H1299 (d) cells. The cells were treated with CG for 48 h, fixed, and stained with PI. The proportion of A549 (e) and NCI-H1299 (f) cells in the sub-G1 phase. Protein expression of cyclin D1, cyclin E, cyclin A, and GAPDH in A549 (g) and NCI-H1299 (h) cells, as determined by western blotting. The data are presented as the mean ± SEM (n = 3). The data were analyzed using one-way ANOVA with Tukey’s HSD test. *, p < 0.05 and **, p < 0.005