Fig. 8

Effect of CA treatment on DNA-binding activity of ARE in HepG2 cells. Cells were treated with 0.1% DMSO (lanes 2 and 4) and with the indicated concentration of CA (lanes 5–7) for 24 h. After treatment with CA, HepG2 cells were incubated with a 0.3 mM concentration of t-BHP for 2 h. Nuclear proteins (8 μg) were isolated, incubated with a labeled ARE sequence and subjected to electrophoretic mobility shift assay. A competition experiment (200-fold excess of unlabeled ARE) was performed with nuclear protein extracted from the non-treated control (lane 2)