Fig. 1
Effects of polysaccharide extract from XJEK on NO content and eNOS expression in Ang II-induced HUVECs. (a): eNOS expression in HUVECs by immunofluorescence analysis (magnification, × 400). (b): NO content in supernatants of HUVECs; (c): eNOS activity in supernatants of HUVECs (ELISA assay); (d): eNOS expression in HUVECs by Western blot analysis and (e) statistical analysis of eNOS protein expression. 1, blank control group; 2, Ang II (10− 5 mol/l) group; 3, Ang II (10− 5 mol/l) + AqE (0.15 mg/ml) group; 4, Ang II (10− 5 mol/l) + AqE (0.3 mg/ml) group; 5, Ang II (10− 5 mol/l) + AqE (0.6 mg/ml) group; 6, Ang II (10− 5 mol/l) + AqE (1.2 mg/ml) group; 7, Ang II (10− 5 mol/l) + XJEK (1.6 mg/ml) group. Data are presented as the mean ± SD (n = 3). **P < 0.01 vs. control group; #P < 0.05, ##P < 0.01vs. Ang II group