Fig. 4

The effects of ALA and AHSO on differentiation of cultured eNSCs toward astrocyte and oligodendrocyte cells. eNSCs dissociated from primary neurospheres were differentiated in 1% FBS-containing medium lacking EGF. Five days after treatment with various concentrations of ALA or AHSO, cells were fixed with 4% PFA and processed by immunocytochemistry. Representative images of double immunofluorescence for astrocytes (GFAP, red) and oligodendrocytes (MBP, green). The blue color indicates nuclear staining (Hoechst). Scale bar = 100 nm