Skip to main content
Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Physalin A regulates the Nrf2 pathway through ERK and p38 for induction of detoxifying enzymes

Fig. 4

Physalin A induces NQO-1 transcription in HepG2 cells. a Viability of physalin A-treated HepG2 cell line. Cells treated with various concentration physalin A for 24 h. b NQO-1 expression was measured using real-time PCR. c ARE transcriptional activity of physalin A-treated HepG2 cells. The cells were treated with 5, 10, 20 μM physalin A for 24 h and cell lysates were used for luciferase assay. d Oligonucleotide pull-down assay in HepG2 cells with ARE element. The cells were treated 20 μM physalin A for 4 h and then harvested to determine ARE-binding activity. e Western blot analysis to assess the expression of Nrf2 and its target genes, NQO-1 and HO-1 in HepG2 cells. The cells were treated with 10 and 20 μM physalin A (*: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001)

Back to article page