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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Effect of Celastrus orbiculatus in inhibiting Helicobacter pylori induced inflammatory response by regulating epithelial mesenchymal transition and targeting miR-21/PDCD4 signaling pathway in gastric epithelial cells

Fig. 6

Over-expression of miR-21 and knockdown of PDCD4 impairs the anti-inflammatory effect of COE. GES-1 cells were pre-transfected with miR-21 precursor, PDCD4 siRNA and then exposed to COE for 24 h. (a) The relative miR-21 level was determined by RT-PCR. (b-c) Cell lysates were prepared to determine the protein level of PDCD4 using western blot analysis. The expression of PDCD4 was determined by western blot. (d) The MSP assay was performed to evaluate the correlation between the PDCD4 methylation status. (e) The anti-inflammatory effect of COE was determined by testing the levels of IL-6, IL-8 and TNF-α. Data are presented as mean ± SD of three separate experiments, *P < 0.05, **P < 0.01, compared to H. pylori infection-induced group, #P < 0.05, ##P < 0.01, compared to transfected with miR-21 precursor or PDCD4 siRNA group.

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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