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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine

Fig. 3

Analysis of transporters and inflammatory factors. a Protein levels of transporters OAT1, OAT3 and URAT1 were determined using immunoblot analysis in kidney cell lines. b After treatment of LLC-PK1 cells with PO and K-25 for 24 h, cell viability was measured using the cell viability assay. c Protein levels of transporters OAT1, OAT3, GLUT9 and URAT1 were determined using ELISA and immunoblot analysis in PO-induced LLC-PK1 cells. d Activation of NGAL and KIM-1 by IL-1α. The protein levels in IL-1α-treated LLC-PK1 cells were detected using ELISA. e LLC-PK1 cells were stimulated with IL-1α (10 ng/mL) with or without pretreatment with K-25 (0.5 mg/mL). f After 24 h-incubation, NGAL and KIM-1 levels were measured using ELISA. Values represent mean ± standard error of the mean. **p < 0.01, compared to the IL-1α control. The statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001) was determined using ANOVA with Bonferroni correction

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