Fig. 2

Inhibition of LPS-induced NO production and IL-1α secretion by K-25. Raw 264.7 cells were pretreated with various concentrations of K-25 for 1 h before LPS (100 ng/mL) treatment. a After 24 h-incubation, the cultures were subjected to cytokine antibody array assay. b After 24 h-incubation, cell viability was measured using the EZ-cytox assay kit. c After 24 h of incubation, NO production was measured using the Griess Reagent System assay. d After 24 h-incubation, the culture medium was assayed using ELISA for IL-1α. Values represent mean ± standard error of the mean. **p < 0.01, compared to the LPS control. The statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001) was determined using ANOVA with Bonferroni correction