Fig. 3

The suppressive effects of Resv on the differentiation of Th17 cells under Th17 specific polarizing conditions. Effects of Tac with or without Resv on CD4⁺ T cells isolated from PBMCs of healthy donors and cultured under Th17 polarizing conditions. (a) The percentage of CD4⁺ T cells producing IL-17 was measured by flow cytometry. (b) The effects of Tac with or without Resv on IL-17⁺ /CD4⁺ T cells, and effects on (c) IL-17 levels (or (e) IL-22) and on the expression of (d) IL-17 (or (f) IL-22) mRNA. *P < 0.05 vs. Th17 polarizing ^†P < 0.05 vs. Tac 1 ng/mL group ^‡P < 0.05 vs. Tac 10 ng/mL group ^§P < 0.05 vs. Tac 1 ng/mL + Resv 25 μM group. Resv, Resveratrol; Tac, tacrolimus