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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: Multi-targeting chemoprevention of Chinese herb formula Yanghe Huayan decoction on experimentally induced mammary tumorigenesis

Fig. 1

Effects of YHHY Decoction on DMBA-induced tumorigenesis and oncogenic myc activation. a. Effect of YHHY Decoction treatment on body weight gain during DMBA-induced mammary tumorigenesis in rats. Each data point indicates mean ± SD. There were 10–18 animals in various groups. No significant difference in body weights was observed among various rat groups at any time point. b. Effect of YHHY Decoction treatment on the occurrence of palpable tumors in the DMBA-induced rats. The length of time before palpable tumor is examined is defined as tumor-free survival time. n = 18 of the YHHY+DMBA group, and n = 14 of the DMBA control group. P = 0.014, Log rank test. c. Histopathology of the tumors detected in the DMBA control and YHHY Decoction treatment groups. DMBA control tumors showed extensive epithelial proliferation (a) and nuclear pleomorphism (b). Tumors treated with YHHY Decoction showed significantly improved cell architecture (c) and almost normal ductal and alveolar structure (d). Scale bar: 50 μm d. Immunohistochemical staining of phospho-c-myc in the mammary glands. Rats’ tissue were harvested two weeks after DMBA induction. The YHHY decoction treatment was initiated 1 week before DMBA induction and lasted for two weeks. Extensive staining of phospho-c-myc was observed in all examined animals in the DMBA group, while none of the animals in the YHHY prevention group showed obviously positive staining (n = 3). Scale bar: 50 μm e. Expressions of phospho-c-myc and total c-myc in 3 individual tumors in the YHHY Decoction treated vs. DMBA control group

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