Fig. 1

Effect of STL and STB on the cell viability, and the level of cleaved PARP and cyclin D1. a and b Cell viability of SW480 cells analyzed by MTT assay. ^*P < 0.05 compared to the cells without (c) The cleaved PARP level analyzed by Western blot analysis in SW480 cells treated with STL and STB for 24 h. Actin was used as loading control. d The protein and mRNA level of cyclin D1 analyzed by Western blot analysis and RT-PCR in SW480 cells treated with STL and STB for 24 h. Actin or GAPDH was used as loading control. The density of protein and mRNA bands was calculated using the software UN-SCAN-IT gel version 5.1 (Silk Scientific Inc. Orem, UT, USA). ^*P < 0.05 compared to the cells without STL and STB. e Proportions of the cycle phase analyzed by flow cytometry in in SW480 cells treated with STL and STB for 24 h. ^*P < 0.05 compared to the cells without STL and STB.