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Table 2 Inhibitory effect of isolated compounds on LPS-induced nitric oxide release from RAW 264.7 cell lines

From: Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

Sample

%Inhibition at various concentration (μg/mL)

(%Viability)

IC50(μg/mL)

0.01

0.1

1

10

50

Crude extract

–

16.65 ± 6.18 (92.51 ± 2.14)

32.42 ± 5.42 (89.44 ± 3.74)

54.64 ± 2.23 (83.49 ± 2.66)

68.38 ± 1.04 (78.82 ± 2.83)

6.06 ± 0.43*

Compound 1

–

2.66 ± 0.23 (84.66 ± 7.91)

3.91 ± 3.03 (89.71 ± 12.08)

14.41 ± 1.71 (84.71 ± 9.65)

69.15 ± 2.49 (80.46 ± 3.10)

40.40 ± 1.32*

Compound 2

–

7.09 ± 6.86 (94.52 ± 10.55)

11.12 ± 9.89 (88.81 ± 8.07)

8.69 ± 6.93 (80.85 ± 7.39)

58.83 ± 3.59 (90.39 ± 9.92)

33.61 ± 5.25*

Compound 3

–

−0.82 ± 3.26 (102.40 ± 2.17)

2.64 ± 0.31 (96.95 ± 0.98)

14.59 ± 3.43 (97.27 ± 4.43)

34.44 ± 3.07 (91.62 ± 3.87)

> 50*

Prednisolone (Positive control)

19.82 ± 3.43 (100.40 ± 12.94)

47.72 ± 6.24 (84.28 ± 4.56)

69.94 ± 3.76 (89.46 ± 2.28)

77.94 ± 8.12 (80.78 ± 2.71)

–

0.11 ± 0.02

  1. Data performed as mean ± SD from triplicate experiments. Mean difference of IC50 data of tested sample and positive control were compared by Student’s t-test. *Significant difference at p-value < 0.05