Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

Table 1 Inhibitory effect of crude ethanolic extract and isolated compounds from Baliospermum montanum root on releasing of β-hexosaminidase from RBL-2H3 cell line

Sample	%Inhibition at various concentraction (μg/ml)					IC₅₀ (μg/ml)
Sample	0.1	1	10	50	100	IC₅₀ (μg/ml)
Crude extract	–	21.59 ± 0.59	81.77 ± 5.94	92.28 ± 1.84	94.13 ± 2.49	4.97 ± 0.43*
Compound 1	−7.88 ± 3.01	4.84 ± 3.46	29.40 ± 2.01	77.74 ± 3.60	–	21.20 ± 2.01
Compound 2	−5.37 ± 2.30	15.05 ± 1.71	30.37 ± 6.70	75.18 ± 6.47	–	26.25 ± 6.06
Compound 3	−14.21 ± 5.67	8.52 ± 2.93	17.38 ± 5.72	60.84 ± 8.98	–	44.42 ± 7.33*
Chlorpheniramine (Positive control)	–	14.89 ± 1.57	36.17 ± 2.84	71.43 ± 11.01	81.27 ± 9.51	17.74 ± 1.61

Data performed as mean ± SD from triplicate experiments. Mean difference of IC₅₀ data of tested sample and positive control were compared by Student’s t-test. * Significant difference at p-value < 0.05

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