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Table 5 Bacterial reverse mutation assay

From: An oral toxicity test in rats and a genotoxicity study of extracts from the stems of Opuntia ficus-indica var. saboten

Test Strain Chemical Treated Dose (μg/plate) Colonies/plate [factor]a
With S9 mix Without S9 mix
TA100 Test article 0 114 ± 13 109 ± 3
5 130 ± 7 [1.1] 115 ± 11 [1.1]
15 98 ± 9 [0.9] 104 ± 0 [1.0]
50 117 ± 13 [1.0] 113 ± 14 [1.0]
150 126 ± 9 [1.1] 115 ± 10 [1.1]
500 116 ± 12 [1.0] 116 ± 3 [1.1]
1500 121 ± 0 [1.1] 121 ± 7 [1.1]
3000 133 ± 4 [1.2] 120 ± 11[1.1]
5000 134 ± 3 [1.2] 104 ± 6 [1.0]
TA1535 Test article 0 12 ± 1 12 ± 2
5 11 ± 1 [0.9] 11 ± 1 [0.9]
15 10 ± 1 [0.8] 12 ± 4 [1.0]
50 12 ± 1 [1.0] 13 ± 3 [1.1]
150 9 ± 1 [0.7] 10 ± 1 [0.8]
500 12 ± 3 [1.0] 14 ± 4 [1.2]
1500 11 ± 1 [0.9] 14 ± 4 [1.2]
3000 9 ± 0 [0.8] 10 ± 1 [0.9]
5000 12 ± 3 [1.0] 11 ± 2 [0.9]
TA98 Test article 0 26 ± 4 24 ± 4
5 27 ± 4 [1.0] 20 ± 1 [0.8]
15 23 ± 2 [0.9] 21 ± 1 [0.9]
50 25 ± 2 [1.0] 26 ± 6 [1.1]
150 27 ± 4 [1.0] 25 ± 1 [1.0]
500 30 ± 2 [1.2] 24 ± 3 [1.0]
1500 27 ± 1 [1.1] 23 ± 1 [1.0]
3000 29 ± 1 [1.1] 23 ± 3 [1.0]
5000 31 ± 4 [1.2] 27 ± 4 [1.1]
TA1537 Test article 0 11 ± 1 9 ± 1
5 10 ± 2 [0.9] 8 ± 0 [0.9]
15 10 ± 1 [0.9] 8 ± 3 [0.9]
50 10 ± 2 [0.9] 9 ± 4 [1.0]
150 11 ± 4 [1.0] 7 ± 2 [0.8]
500 14 ± 4 [1.3] 8 ± 2 [0.9]
1500 12 ± 2 [1.1] 7 ± 1 [0.8]
3000 9 ± 0 [0.9] 7 ± 1 [0.8]
5000 9 ± 0 [0.9] 7 ± 1 [0.8]
E. coli WP2 uvrA Test article 0 29 ± 0 22 ± 4
5 23 ± 1 [0.8] 27 ± 1 [1.2]
15 24 ± 4 [0.8] 23 ± 4 [1.1]
50 29 ± 4 [1.0] 23 ± 3 [1.1]
150 26 ± 1 [0.9] 21 ± 3 [1.0]
500 25 ± 1 [0.9] 26 ± 2 [1.2]
1500 25 ± 6 [0.9] 24 ± 4 [1.1]
3000 26 ± 0 [0.9] 21 ± 4 [1.0]
5000 23 ± 1 [0.8] 26 ± 8 [1.2]
Positive controls (μg/plate)   
TA100 2-AA 1.0 1588 ± 14 [13.7]  
TA1535 2-AA 2.0 183 ± 2 [15.2]  
TA98 B[a]P 1.0 159 ± 6 [6.2]  
TA1537 2-AA 1.0 239 ± 1 [22.8]  
WP2 uvrA 2-AA 6.0 143 ± 11 [4.9]  
TA100 SA 0.5   408 ± 5 [3.7]
TA1535 SA 0.5   286 ± 6 [24.8]
TA98 2-NF 2.0   208 ± 11[8.7]
TA1537 ICR-191 0.5   59 ± 1 [6.9]
WP2 uvrA 4NQO 0.5   167 ± 9 [7.7]
  1. Test article: Extract of 70% extract of palm cactus stem
  2. aTwo plate/dose were used. No. of colonies of treated plate/No. of colonies of the negative control plate
  3. Abbreviations: 2-AA: 2-aminoanthracene, SA: sodium azide, B[a]P: benzo[a]pyrene, ICR-191: acridine mutagen ICR 191, 4NQQ: 4-nitroqinoline N-oxide, 2-NF: 2-Nitrofluorene