Table 1 Primers for qRT-PCR
Target gene | Primer(5′-3′) | GenBank Accession |
|---|---|---|
PPARγ | F:5′- ATGACAGACCTCAGGCAGATTG − 3′ | XM_006237009 |
R:5′- GTCAGCGACTGGGACTTTTCT − 3′ | ||
PLIN1 | F:5′- ATGAGGAGGAAGAAGAAGAAGAGTC -3′ | XM_008759499 |
R:5′- TCAGGGCATCGGATAGGGA − 3′ | ||
OSX | F:5′- GTTCACCTGTCTGCTCTGCT − 3’ | XM_006242390 |
R:5′- TTGGCTTCTTCTTCCCCGAC -3’ | ||
ALP | F:5′- CCCACAAGAGCCCACAATGG − 3’ | XM_006239136 |
R:5′- CGGAAGTGAGGCAGGTAGCAAA − 3’ | ||
actin | F:5′- ACCGTGAAAAGATGACCCAGAT − 3’ | NM_031144 |
R:5′- CCAGAGGCATACAGGGACAACA − 3’ | ||
OCN | F:5′- GAGGGCAGTAAGGTGGTGAATAG − 3’ | NM_013414.1 |
R:5′- AAGCCAATGTGGTCCGCTAG-3’ | ||
BSPII | F:5′- CAACGGCACCAGCACCAA-3’ | NM_012587.2 |
R:5′- TCGTATTCTTCCCCATACTCAACC-3’ | ||
COL2A1 | F:5’-TAAAACCCTCAACCCCAAAACA-3’ | NM_012929.1 |
R:5′- ATCAGGTCAGGTCAGCCATTCA-3’ |