Fig. 3

Effect of different concentrations of Osteoking on alkaline phosphatase expression in rbMSCs. a Alkaline phosphatase (ALP) staining of rat bone marrow mesenchymal stem cells (rbMSCs) cultured in mesenchymal stem cell (MSC) medium or osteoblast maturation medium with different concentrations of Osteoking after 12 and 20 days. D = day, X = dilution multiple of Osteoking. b Gene expression analysis for ALP of rbMSCs cultured in osteoblast maturation medium with different concentrations of Osteoking after 12 and 20 days by qRT-PCR., ***p < 0.001 vs. control; n = 5