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Table 5 Intraday and interday precision and accuracy of HPLC-PDA method with mixture of polyphenolic compounds injected at highest concentration

From: Development of aqueous-based multi-herbal combination using principal component analysis and its functional significance in HepG2 cells

Precisiona and accuracyb Polyphenolic compounds
GA1 CAT2 ChA3 ECAT4 CA5 UMB6 CoA7 RUT8 EA9 QUE10 KAE11
Intraday (n = 9)
 Day 1
  Average area 9,498,108 1,587,143 2,536,193 2,519,193 4,619,983 1,549,082 9,747,582 2,579,650 1,499,363 2,429,518 782,051
  ±SD 15,273 15,413 18,257 20,705 34,228 15,372 66,280 14,123 11,745 8846 2835
  %RSD 0.161 0.971 0.720 0.822 0.741 0.992 0.680 0.547 0.783 0.364 0.363
  %Recovery 99.9 99.8 99.7 99.4 99.7 99.5 99.7 99.8 99.0 99.9 99.9
 Day 2
  Average area 9,488,221 1,589,559 2,529,570 2,519,830 4,625,180 1,551,030 9,751,220 2,579,215 1,496,578 2,428,618 782,443
  ±SD 24,151 12,751 24,681 22,101 37,601 16,210 68,152 12,489 12,930 9764 3956
  %RSD 0.255 0.802 0.976 0.877 0.813 1.05 0.699 0.484 0.864 0.402 0.505
  %Recovery 99.8 99.9 99.4 99.4 99.8 99.6 99.8 99.8 98.8 99.8 100
 Day 3
  Average area 9,501,364 1,586,062 2,541,010 2,522,814 4,629,476 1,548,746 9,743,617 2,582,113 1,503,104 2,432,157 782,007
  ±SD 16,429 14,829 24,319 24,128 34,215 14,814 74,613 12,056 13,689 7955 2245
  %RSD 0.173 0.935 0.957 0.956 0.739 0.957 0.766 0.467 0.911 0.327 0.287
  %Recovery 99.9 99.7 99.9 99.5 99.9 99.4 99.7 99.9 99.2 100 99.9
Interday (n = 27)
 3 Days with 9 replicates per day
  Average area 9,495,898 1,587,588 2,535,591 2,520,612 4,624,880 1,549,619 9,747,473 2,580,326 1,499,682 2,430,098 782,167
  ±SD 14,065 14,331 25,336 23,613 36,348 16,041 71,681 13,849 13,280 9962 3142
  %RSD 0.148 0.903 0.999 0.937 0.786 1.04 0.735 0.537 0.852 0.410 0.401
  %Recovery 99.8 99.8 99.6 99.4 99.8 99.5 99.7 99.9 99.0 99.9 100
  1. 1Gallic acid; 2Catechin; 3Chlorogenic acid; 4Epicatechin; 5Caffeic acid; 6Umbelliferone; 7p-Coumaric acid; 8Rutin; 9Ellagic acid; 10Quercetin; 11Kaempferol; a%RSD; b%Recovery