Skip to main content

Table 5 Intraday and interday precision and accuracy of HPLC-PDA method with mixture of polyphenolic compounds injected at highest concentration

From: Development of aqueous-based multi-herbal combination using principal component analysis and its functional significance in HepG2 cells

Precisiona and accuracyb

Polyphenolic compounds

GA1

CAT2

ChA3

ECAT4

CA5

UMB6

CoA7

RUT8

EA9

QUE10

KAE11

Intraday (n = 9)

 Day 1

  Average area

9,498,108

1,587,143

2,536,193

2,519,193

4,619,983

1,549,082

9,747,582

2,579,650

1,499,363

2,429,518

782,051

  ±SD

15,273

15,413

18,257

20,705

34,228

15,372

66,280

14,123

11,745

8846

2835

  %RSD

0.161

0.971

0.720

0.822

0.741

0.992

0.680

0.547

0.783

0.364

0.363

  %Recovery

99.9

99.8

99.7

99.4

99.7

99.5

99.7

99.8

99.0

99.9

99.9

 Day 2

  Average area

9,488,221

1,589,559

2,529,570

2,519,830

4,625,180

1,551,030

9,751,220

2,579,215

1,496,578

2,428,618

782,443

  ±SD

24,151

12,751

24,681

22,101

37,601

16,210

68,152

12,489

12,930

9764

3956

  %RSD

0.255

0.802

0.976

0.877

0.813

1.05

0.699

0.484

0.864

0.402

0.505

  %Recovery

99.8

99.9

99.4

99.4

99.8

99.6

99.8

99.8

98.8

99.8

100

 Day 3

  Average area

9,501,364

1,586,062

2,541,010

2,522,814

4,629,476

1,548,746

9,743,617

2,582,113

1,503,104

2,432,157

782,007

  ±SD

16,429

14,829

24,319

24,128

34,215

14,814

74,613

12,056

13,689

7955

2245

  %RSD

0.173

0.935

0.957

0.956

0.739

0.957

0.766

0.467

0.911

0.327

0.287

  %Recovery

99.9

99.7

99.9

99.5

99.9

99.4

99.7

99.9

99.2

100

99.9

Interday (n = 27)

 3 Days with 9 replicates per day

  Average area

9,495,898

1,587,588

2,535,591

2,520,612

4,624,880

1,549,619

9,747,473

2,580,326

1,499,682

2,430,098

782,167

  ±SD

14,065

14,331

25,336

23,613

36,348

16,041

71,681

13,849

13,280

9962

3142

  %RSD

0.148

0.903

0.999

0.937

0.786

1.04

0.735

0.537

0.852

0.410

0.401

  %Recovery

99.8

99.8

99.6

99.4

99.8

99.5

99.7

99.9

99.0

99.9

100

  1. 1Gallic acid; 2Catechin; 3Chlorogenic acid; 4Epicatechin; 5Caffeic acid; 6Umbelliferone; 7p-Coumaric acid; 8Rutin; 9Ellagic acid; 10Quercetin; 11Kaempferol; a%RSD; b%Recovery