Fig. 6
a Effect of VCN-2 hydrocolloid film on expression of mediators after 14 days treatment was detected by Western blotting. Down-regulation of various anti-inflammatory and up-regulation of expression of several wound healing markers in skin tissues were observed. β-actin acted as control marker. b Effect of VCN-2 film on expression of mediators after 14 days topically treatment was detected by immunoblotting. A graph represented densitometry analysis results of the effect of VCN-2 on proteins expression proinflammatory mediators (c) anti-HIF1α, (d) MMP-9, and (e) VEGF and TGF-β. All data are expressed as mean ± SEM. ### p < 0.001 represented statistical difference diabetic group compared to normal group; *** p < 0.001 represented statistical difference treatment groups compared to the diabetic group