Skip to main content
Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Suppression of lung inflammation by the ethanol extract of Chung-Sang and the possible role of Nrf2

Fig. 4

The effect of eCS on Nrf2 activity. a Nuclear proteins were fractionated from RAW 264.7 cells treated with various amounts of eCS for 16 h and then analyzed by immunoblotting for Nrf2. b The relative amounts of nuclear Nrf2 were calculated over Lamin A/C using ImageJ. ***P was less than 0.0001, compared to the untreated control. Data are presented as the mean ± SEM of 3 measurements, and a representative of 3 independent experiments is shown. c HEK 293 cells were transfected with V5-Nrf2, HA-Ub, and Flag-Keap1 and then treated with eCS for 16 h with or without MG132 (5 μM, 2 h). V5-Nrf2 in the total cell lysate was precipitated with the anti-V5 antibody and the precipitant was analyzed with the anti-HA antibody to reveal the ubiquitinated Nrf2. One-tenth of total cell lysate was analyzed for Flag (Keap1), V5 (Nrf2), and β-actin, as inputs. d Similarly, HEK 293 cells were transfected with V5-Nrf2 and HA-Ub, along with MG132 (5 μM, 2 h). V5-Nrf2 in the total cell lysate was precipitated with an isotypic IgG or the anti-V5 antibody. The precipitants were analyzed with the anti-HA antibody to reveal the ubiquitinated Nrf2. One-tenth of total cell lysate was analyzed for V5-Nrf2 as in a input

Back to article page