Table 1 The primer details used for real-time PCR analysis
Genes | Forward sequence (5′ to 3′) | Reverse sequence (5′ to 3′) | Amplicon length (bp) | GenBank accession number |
|---|---|---|---|---|
eNos | GGA TTC TGG CAA GAC CGA TTA C | GGT GAG GAC TTG TCC AAA CAC T | 159 | NM_021838.2 |
Vcam-1 | GGA GCC TGT CAG TTT TGA GAA TG | TTG GGG AAA GAG TAG ATG TCC AC | 105 | NM_012889.1 |
TNF-α | ACT GAA CTT CGG GGT GAT TG | GCT TGG TGG TTT GCT ACG AC | 153 | NM_012675.3 |
Il-6 | ATATGTTCTCAGGGAGATCTTGGAA | GTGCATCATCGCTGTTCATACA | 80 | NM_012589.2 |
Cd36 | AGGAAGTGGCAAAGAATAGCAG | ACAGACAGTGAAGGCTCAAAGA | 163 | NM_031561.2 |
Mcp-1 | GGC CTG TTG TTC ACA GTT GCT | TCT CAC TTG GTT CTG GTC CAG T | 264 | NM_031530.1 |
Gapdh | GCA AGT TCA ACG GCA CAG | GCC AGT AGA CTC CAC GAC AT | 140 | NM_017008.4 |