Fig. 7

JPYSF decreases mitochondrial fission and increases mitochondrial fusion. a Representative Western blot images show increased Drp-1, decreased Mfn-2 and OPA-1 expression in the 5/6 Nx group, which were restored by JPYSF treatment. b-d Densitometric analysis of Drp-1, Mfn-2, and OPA-1 protein expression normalized to GAPDH content. e Representative immunofluorescence images of Drp-1, Mfn-2, and OPA-1. Green corresponds to interest proteins, and blue corresponds to nuclear staining. All images are shown at identical magnification, × 200, scale bar = 100 μm. Data are presented as the means ± SEM, n = 6 rats per group. (*P < 0.05, **P < 0.01 compared with the sham group; ^##P < 0.01 compared with the 5/6 Nx group)