Fig. 6

JPYSF increases mitochondrial biogenesis in 5/6 Nx rats. a Representative Western blot images show decreased PGC-1α, NRF-1, and TFAM expression in the 5/6 Nx group, which were increased by JPYSF treatment. b-d Densitometric analysis of PGC-1α, NRF-1, and TFAM protein expression normalized to GAPDH content. e Representative immunofluorescence images of PGC-1α and TFAM. Green corresponds to interest proteins, and blue corresponds to nuclear staining. All images are shown at identical magnification, × 200, scale bar = 100 μm. Data are presented as the means ± SEM, n = 6 rats per group. (*P < 0.05, **P < 0.01 compared with the sham group; ^#P < 0.05, ^##P < 0.01 compared with the 5/6 Nx group)