Table 1 Assay ID of the inventory primers and probes and the sequence of the self-designed primers used for qRT-PCR
Gene | Accession number | Assay ID or primer sequence |
|---|---|---|
Ucp1 | NM_009463.3 | Mm01244861_m1 |
Npy | NM_023456.3 | F: CAGAACAAGGCTTGAAGACC C R: GCAGACTGGTTTCAGGGGAT |
AgRP | NM_007427.3 | F: GAGTTCCCAGGTCTAAGTCTGAATG R: ATCTAGCACCTCCGCCAAAG |
Pomc | NM_008895 | F: CCCGCCCAAGGACAAGCGTT R: CTGGGCCCTTCTTGTGCGCGT |