Fig. 2

Cardamonin induced cell autophagy. SKOV3 cells were treated with cardamonin (5, 20 μM), rapamycin (0.1 μM) and 2-DG (10 mM) for 48 h, respectively. a MDC stain was applied to detect the level of autophagy. b The quantitative results of fluorescent intensity. c Expression of LC3 and LAMP1 was detected by Western blot. d, e Quantification of LC3-II and LAMP1 by densitometry of Western blot performed above. ^*p < 0.05, ^**p < 0.01 vs. control. Data were represented as mean ± SD, n = 3