Skip to main content

Table 3 Antibacterial percentage cytosolic lactate dehydrogenase assay and percentage accumulation of R6G inside the cell after exposure to glucose, plant extract and standard inhibitor bererine

From: In vitro antibacterial and cytotoxic activity of leaf extracts of Centella asiatica (L.) Urb, Warburgia salutaris (Bertol. F.) Chiov and Curtisia dentata (Burm. F.) C.A.Sm - medicinal plants used in South Africa

Percentage accumulation and release

 

Centella asiatica

Curtisia dentate

Warburgia salutaris

Control

Test organisms

 

Methanol

Ethyl

Acetate

Acetone

Water

Methanol

Ethyl

Acetate

Acetone

water

Methanol

Ethyl

Acetate

Acetone

water

berberine

E. avium

LDHa

0.68

–

0

–

8.1

–

0.7

–

2.4

–

0

–

53

R6Gb

11

31

10

–

30

–

24

–

3

–

21

–

S. aureus

LDHa

0

0.3

3.6

–

7.2

2.1

5.4

–

0.8

0

36.4

–

87

R6Gb

20

42

5

–

9

35

25

–

53

20

6

–

S. agalactiae

LDHa

–

–

–

–

–

–

0.7

–

0.5

–

1.4

–

76

R6Gb

–

–

–

–

–

–

14

–

15

–

3

–

B. cereus

LDHa

–

0.8

4.3

–

1.4

–

–

–

15.1

–

0.3

–

85

R6Gb

–

39

13

–

12

–

–

–

10

–

8

–

E. hirae

LDHa

–

–

–

–

–

–

–

–

–

0

0.1

–

75

R6Gb

–

–

–

–

–

–

–

–

–

24

9

–

E. faecalis

LDHa

–

–

–

–

–

–

–

–

–

–

1.0

–

67

R6Gb

–

–

–

–

–

–

–

–

–

–

6

–

E. gallinarium

LDHa

0

–

–

–

–

–

5.1

–

1.6

–

–

–

55

R6Gb

9

–

–

–

–

–

18

–

23

–

–

–

E. Coli

LDHa

0.8

–

18.6

–

1.4

0

18.6

–

25.4

1.34

4.3

–

92

R6Gb

11

–

45

–

31

53

39

–

35

24

11

–

P. aeruginosa

LDHa

–

–

–

–

–

–

4.4

–

3.3

–

0.9

–

64

R6Gb

–

–

–

–

–

–

51

–

24

–

7

–

P. mirabilis

LDHa

–

–

–

–

0.7

–

–

–

–

–

–

–

41

R6Gb

–

–

–

–

18

–

–

–

–

–

–

–

P. vulgaris

LDHa

–

–

0

–

–

–

–

–

–

–

–

–

50

R6Gb

–

–

6

–

–

–

–

–

–

–

–

–

K. pneumonia

LDHa

–

–

–

–

–

–

–

–

4.7

0

–

–

79

R6Gb

–

–

–

–

–

–

–

–

 

6

–

–

Acinetobacter

Calcaoceuticals

Anitratus

LDHa

–

–

–

–

–

–

–

–

–

–

0

–

66

R6Gb

–

–

–

–

–

–

–

–

–

–

23

–

S. typhi

LDHa

–

–

–

–

–

–

–

–

–

–

0

–

45

R6Gb

–

–

–

–

–

–

–

–

–

–

11

–

  1. aLDH release (membrane damage) activity % LDH Release in comparison to Triton X-100; bValues of drug accumulation in the presence of glucose were taken as the control, − = not determined. (P ≤ 0.05)