Fig. 5
Effects of BPRBE on induction of IFN-β production through IRF3 activation in S. Typhimurium-infected macrophages. a Phospho-IRF3 protein (p-IRF3) expression profiles assessed by Western blot analysis. RAW 264.7 cells (1 × 105) were treated with BPRBE and NPRBE for 24 h and infected with S. Typhimurium and lysed. The phospho-form of IRF3 in the cell lysates was identified using Western blotting. b IFN-β release from S. Typhimurium-infected macrophages pre-treated with BPRBE and NPRBE was determined using an ELISA kit. Data are expressed as the mean ± SD of triplicate experiments. Bars sharing a common letter are not significantly different at p < 0.05