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Table 4 Histopathology and histomorphometry of the femur in sham-operated and OVX mice: Trabecular Bones

From: Anti-osteoporotic effects of mixed compositions of extracellular polymers isolated from Aureobasidium pullulans and Textoria morbifera in ovariectomized mice

Items
Groups
Left femur tissues
TV/BV Tbn Tbl Tbt
Controls
 Sham 39.33 ± 5.16 18.63 ± 2.56 967.87 ± 131.16 68.48 ± 10.39
 OVX 11.33 ± 2.10e 3.38 ± 1.19e 318.15 ± 76.18a 35.91 ± 10.13e
Reference (2.5 mg/kg)
 RES 43.99 ± 7.02g 27.50 ± 5.53eg 828.28 ± 109.33ab 36.51 ± 10.81e
Single formula (200 mg/kg)
 EAP 24.75 ± 2.46eg 10.50 ± 1.60eg 594.88 ± 94.79ab 49.42 ± 2.60eg
 TM 22.77 ± 3.11eg 8.75 ± 2.96eg 656.09 ± 127.21ab 48.86 ± 3.98eg
Mixed formula - EAP:TM (200 mg/kg)
 1:1 24.38 ± 3.73eg 9.88 ± 2.23eg 551.50 ± 64.08ab 48.70 ± 5.40fh
 1:3 23.68 ± 3.09eg 10.25 ± 1.49eg 647.64 ± 147.56ab 49.76 ± 7.89eh
 1:5 22.41 ± 3.53eg 9.63 ± 1.85eg 635.86 ± 99.62ab 52.72 ± 10.21eg
 1:7 23.39 ± 3.11eg 9.38 ± 1.92eg 646.56 ± 62.11ab 49.70 ± 5.97eg
 1:9 23.66 ± 3.97eg 10.88 ± 2.03eg 623.38 ± 86.57ab 52.38 ± 8.86eg
 3:1 35.42 ± 4.00gij 17.88 ± 1.96gij 913.57 ± 151.07bcd 61.78 ± 6.26hij
 5:1 25.06 ± 2.44eg 10.13 ± 1.25eg 663.59 ± 89.99ab 50.78 ± 6.69eg
 7:1 24.39 ± 2.83eg 10.00 ± 2.00eg 633.02 ± 77.63ab 50.30 ± 6.14eg
 9:1 23.79 ± 2.56eg 10.63 ± 2.67eg 600.25 ± 96.45ab 48.45 ± 4.79eg
  1. OVX Bilateral ovariectomy, RES Risedronate sodium, EAP Exopolymers purified from A. pullulans SM2001, TM T. morbifera leaf extracts, (EAP:TM) Mixed formula consisted of EAP and TM (g/g), TV/BV Trabecular bone volume (%), Tbn Trabecular bone number (Numbers/epiphyseal), Tbl Trabecular bone length (Longitudinal thickness; μm), Tbt Trabecular bone thickness (Cross thickness; μm)
  2. Values are expressed mean ± S.D. of eight mice
  3. ap < 0.01 as compared with sham control by LSD test
  4. bp < 0.01 as compared with OVX control by LSD test
  5. cp < 0.01 as compared with EAP single formula treated mice by LSD test
  6. dp < 0.01 as compared with TM single formula treated mice by LSD test
  7. ep < 0.01 and fp < 0.05 as compared with sham control by MW test
  8. gp < 0.01 and hp < 0.05 as compared with OVX control by MW test
  9. ip < 0.01 as compared with EAP single formula treated mice by MW test
  10. jp < 0.01 as compared with TM single formula treated mice by MW test