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Table 7 Effect of pure compounds on superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils

From: Study of the anti-allergic and anti-inflammatory activity of Brachychiton rupestris and Brachychiton discolor leaves (Malvaceae) using in vitro models

Sample Superoxide anion generationa Elastase releasea
IC50 (μΜ)b IC50 (μΜ)b
β-amyrin acetate (1) > 10 > 10
scopoletin (4) > 10 > 10c
β-sitosterol-3-O-β-D-glucoside (5) > 10 > 10
dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucoside (6) > 10 > 10
dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucoside (7) >1d >1d
genistein 1.16 ± 0.12 21.51 ± 6.50
  1. aIC50 values, results are presented as mean ± SD (n = 3–4), compared with the control value (formyl-methionyl-leucyl-phenylalanine/cytochalasin B, fMLF/CB)
  2. bConcentration necessary for 50% inhibition (IC50)
  3. cScopoletin (4) exerted significant inhibitory activity in elastase release assay (22.8 ± 15.3%, *p < 0.05) at 10 μM
  4. dDihydrodehydrodiconiferyl alcohol 9-O-β-D-glucoside (10) was used at the final concentration of 1 μM due to solubility issues