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Table 6 Anti-allergic activity of compounds isolated from B. rupestris

From: Study of the anti-allergic and anti-inflammatory activity of Brachychiton rupestris and Brachychiton discolor leaves (Malvaceae) using in vitro models

Sample % viability, RBL-2H3a % inhibition of A23187-induced β-hexosaminidase releaseb
100 μM 500 μM 10 μM 100 μM 500 μM
β-amyrin acetate (1) 98.3 ± 2.9 c 0.3 ± 0.6 0.7 ± 1.2 c
scopoletin (4) 96.0 ± 4.0 93.7 ± 6.5 3.7 ± 4.7 13.7 ± 8.0 23.0 ± 8.0**d
β-sitosterol-3-O-β-D-glucoside (5) 96.0 ± 1.0 c 5.0 ± 5.6 7.7 ± 3.8 c
dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucoside (6) 97.3 ± 2.5 e 7.0 ± 10.4 12.7 ± 7.6 e
dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucoside (7) 97.7 ± 2.1 95.3 ± 4.2 3.3 ± 4.2 16.3 ± 5.5* 18.0 ± 8.7*
  1. A mixture of β-sitosterol (2) and stigmasterol (3) was toxic towards RBL-2H3 cells at the concentration of 200 μg/mL (73.7 ± 11.7% viability) and 100 μg/ml (78.7 ± 11.6% viability) and inactive at the concentration of 10 μg/mL (5.0% ± 5.0% inhibition) in A23187-induced degranulation assay
  2. aThe cytotoxicity of samples to RBL-2H3 was evaluated using MTT viability assay; results are presented as mean ± SD (n = 3)
  3. bDexamethasone (10 nM) was used as the positive control and inhibited 93.7 ± 1.5%** of A23187-induced β-hexosaminidase release in RBL-2H3 cells. Results are presented as mean ± SD (n = 3); *p < 0.05, **p < 0.001 compared with the control value (A23187 only)
  4. cPrecipitate was formed upon the addition into the medium at the concentration of 500 μM, therefore the result could not be justified
  5. dScopoletin (500 μM) exerted 30.0 ± 7.1% inhibition of antigen-induced β-hexosaminidase release (mean ± SD, n = 2)
  6. eDihydrodehydrodiconiferyl alcohol 4-O-β-D-glucoside was not tested at the concentration of 500 μM, however, it was nontoxic towards RBL-2H3 cells (96.0 ± 6.9% viability) and inactive in A23187-induced degranulation assay (10.0 ± 4.6% inhibition) at the concentration of 200 μM