Skip to main content

Table 3 Effect of the total extracts and fractions of B. rupestris and B. discolor on superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils

From: Study of the anti-allergic and anti-inflammatory activity of Brachychiton rupestris and Brachychiton discolor leaves (Malvaceae) using in vitro models

Sample Superoxide anion generationa Elastase releasea
IC50 (μg/mL)b IC50 (μg/mL)b
BRT 4.92 ± 1.47 3.82 ± 0.55
BRH 5.69 ± 0.80 3.73 ± 1.16
BRD 2.99 ± 0.73 1.98 ± 1.54
BRE 6.25 ± 3.10 2.71 ± 0.79
BRR 3.01 ± 1.91 > 10c
BDT 4.73 ± 0.97 5.37 ± 1.23
BDH 6.25 ± 2.18 6.04 ± 2.32
BDD 0.78 ± 0.29 1.57 ± 0.84
BDE 5.22 ± 1.35 2.95 ± 1.08
genistein 0.41 ± 0.09 4.41 ± 1.99
  1. aIC50 values, results are presented as mean ± SD (n = 3), compared with the control value (formyl-methionyl-leucyl-phenylalanine/cytochalasin B, fMLF/CB)
  2. bConcentration necessary for 50% inhibition (IC50)
  3. cBRR exerted significant inhibitory activity in superoxide anion generation (49.6 ± 2.9%, **p < 0.001) at 10 μg/mL. BRT B. rupestris total methanol extract, BRH B. rupestris n-hexane fraction, BRD B. rupestris dichloromethane fraction, BRE B. rupestris ethyl acetate fraction, BRR B. rupestris remaining MeOH(aq) fraction, BDT B. discolor total methanol extract, BDH B. discolor n-hexane fraction, BDD B. discolor dichloromethane fraction, BDE B. discolor ethyl acetate fraction