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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Acanthus ebracteatus leaf extract provides neuronal cell protection against oxidative stress injury induced by glutamate

Fig. 3

Protective effect of AE leaf extracts against glutamate-induced apoptotic cell death in HT22 cells. (a) Representative flow cytometric scatter plots of annexin V-FITC and PI staining in untreated HT22 cells (control), or cells exposed to 5 mM glutamate alone or glutamate combined with either AEH or AEE at 50 μg/mL for 18 h. (b) The percentages of apoptotic cells in each treatment group calculated as the sum of annexin V-positive/PI-negative cells (early-stage apoptosis, lower right quadrant) plus annexin V/PI double-positive cells (late-stage apoptosis, upper right quadrant). Data are expressed as the means ± SD, ###P < 0.001 vs. control; ***P < 0.001 vs. glutamate alone

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