Fig. 3
The effect of SC on TRAF6 protein expression in RANKL-stimulated RAW 264.7 cells. RAW 264.7 cells (8 × 104 per well) were incubated with 100 ng/mL RANKL or both RANKL and SC (0.6, 6 and 60 μg/mL). After 7 days, total protein was then isolated and protein expression levels were evaluated by Western blot assay. Data are represented as the means ± S.E.M. of three independent experiments. # p < 0.05 compared with non-treated cells. * p < 0.05 and ** p < 0.01 compared with only RANKL-treated cells