Fig. 4

Effect of ES on the MAPK signaling pathway in P. acnes-indcued THP-1 cells. THP-1 cells were pre-treated with 0.1, 1 and 10 μg/ml of ES for 1 hrs incubation and then stimulated with P. acnes for 1 hrs incubation. (a) Western blot analysis shows that phosphorylation of p38, ERK and JNK is suppressed by ES treatment. (b) MAPKs/β-actin ratio were determined by densitometry. Values represent mean ± S.E.M. Data were analyzed by Tukey post hoc test ( *P < 0.05 versus non-treatment and **P < 0.05 versus P.acnes alone)