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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Sotetsuflavone inhibits proliferation and induces apoptosis of A549 cells through ROS-mediated mitochondrial-dependent pathway

Fig. 4

Determination of reactive oxygen species in A549 cells by sotetsuflavone. a Fluorescence image analysis of sotetsuflavone inducing apoptosis in A549 cells stained by DCFH-DA(100 × magnification). b Average fluorescence intensity of ROS was analyzed by flow cytometry after DCFH-DA staining. c Effect of sotetsuflavone on reactive oxygen species in A549 cells. d, e The experiment was divided into five groups, which were respectively added with 0, 64, 128, 128, 0 μmol/L sotetsuflavone, then, 10 mM NAC solution was added in groups 4 and 5, respectively, thereby became 0(control), 64, 128, 128 + NAC and NAC groups. and the images of fluorescent microscopic analysis sotetsuflavone and NAC respectively inducing apoptosis in A549 cells stained with hoechst 33,258 staining. f The experimental group was the same as Fig. 4d, e. and the result showed that inhibitory effect of sotetsuflavone on proliferation of A549 cells. The results from three independent experiments were expressed as mean ± SD compared with the control group, *P < 0.01, ***P < 0.001

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