Fig. 3

The phagocytic capacity of dendritic cells towards CFSE-labeled tumor debris and FITC-dextran. a Flow cytometric analysis indicates the percentage of DCs population with positive uptake of CFSE stained tumor cell remnants in (a) iDCs, (b) HCT 116 treated-DCs and (c) MCF-7 treated-DCs. b Identification of DCs as high HLA-DR⁺ population for (a) unloaded iDCs, (b) HCT 116-treated-DCs and (c) MCF-7-treated-DCs. c Representative FACs analysis data for FITC-dextran uptake by (a) iDCs, (b) HCT 116-TLY-pulsed-DCs, (c) MCF-7-TLY pulsed-DCs, and (d) LPS–stimulated only DCs. d The figure represents the statistically transformed data. ** Indicates significant difference (** P < 0.01) for TLY-treated-DCs versus iDCs. ## Indicated significant difference (## P < 0.01) of TLY-DCs versus LPS-stimulated DCs. The data are representative of three experiments comprising three different healthy donors and analyzed by one-way ANOVA followed by Dunnett’s test. iDCs, immature dendritic cell; TLY, tumor lysate; CFSE, Carboxy-fluorescein succinimidyl ester