Fig. 2

Generation of tumor lysate and monocyte-derived dendritic cells. a Flow cytometric analysis of the apoptotic effect of 80% ethanol extract of Phyllanthus amarus on (i) HCT-116 and (ii) MCF-7. b Characterization of the generated immature monocyte-derived DCs ex vivo at day 6 (a) microscopically, (b) representative gating strategy, and (c-f) flow cytometric analysis of HLA-DR⁺, CD 83⁺, CD 14⁻ and CD 86⁺ expression by iDCs. c The cell viability assay of DCs treated with PA generated tumor lysate (HCT 116 and MCF-7 TLY) by trypan blue exclusion method after 48 h. The data are representative of three experiments comprising three different healthy donors and analyzed by one-way ANOVA followed by Dunnett’s test. ***P < 0.001. Mean ± SEM. are shown. DCs, Dendritic cells; PA, Phyllanthus amarus; Ann V, Annexin V; PI, Propidium iodide