Fig. 4From: Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide)Cell morphology changes of Trypanosoma cruzi by fluorescent and optical microscopy. a Cell morphology, mitochondrial membrane potential, nuclear and kinetoplast DNA of T. cruzi epimastigotes after treatment with essential oils, terpenes, or BNZ. b DNA fragmentation analysis by TUNEL assay on T.cruzi epimastigotes treated with terpenes. The preserved parasitic DNA was visualized with a blue HOECHST fluorescent probe (negative TUNEL) and the free DNA strands were observed in green (positive TUNEL). aDIC: Differential Interference Contrast Microscopy; bNT: No Treatment; cCarOx: caryophyllene oxide; dLimo: limonene; eBNZ: Benznidazole; fPC: Positive control: DAPI: cells stained with DAPI nuclear fluorescent stain observed in UV filter. MitoTracker: cells stained with MitoTracker Red CMXRos stain observed in an Excitation/Emission 579/599 (nm) filter. Photographs are representative of 10 observed fieldsBack to article page