Fig. 4

Effects of corylin on the activation of NLRP3 inflammasome by LPS-activated BV2 cells. BV2 cells were incubated with various doses of corylin for 1 h, and then treated with LPS (1 μg/mL) for 24 h or 48 h. a The level of IL-1β in the cell culture supernatant was examined by ELISA (***: p < 0.001 vs. LPS alone). b The expression levels of NLRP3 and ASC were examined by Western blot. Blots are representative blots. c, d Quantitation was performed on three independent experiments and presented as the means ± SD. The expression of β-actin was used as an internal control. The relative ratios of NLRP3/β-actin and ASC/β-actin are shown (*: p < 0.05; **: p < 0.01 vs. LPS alone). e The expression levels of pro-caspase-1, mature caspase-1 (p20), pro-IL-1β, and mature IL-1β (p17) were examined by Western blot. Blots are representative blots. Quantitation was performed on three independent experiments and presented as the means ± SD. The relative ratio of f mature caspase-1 (p20)/immature caspase-1 and g mature IL-1β (p17)/immature IL-1β are shown (*: p < 0.05; **: p < 0.01 vs. LPS alone)