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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Ginkgolide B inhibits platelet and monocyte adhesion in TNFα-treated HUVECs under laminar shear stress

Fig. 4

Ginkgolide B suppressed VCAM-1, VE-cadherin, and Cx43 expression in TNFα-treated HUVECs that presented monocyte adhesion under different conditions of laminar shear stress. HUVECs were incubated with ginkgolide B (0.6 mg/ml) or aspirin (1 mM) for 12 h, and then TNFα (20 ng/ml) was added for another 2 h. The monocyte suspension was then added to the input well at 1 and 9 dyn/cm2 of laminar shear stress for 45 min. Unadhered monocytes were removed by the addition of PBS buffer for 2 min. Lysates were then collected by the addition of lysis buffer. Protein expression was analyzed by Western blot. a Ginkgolide B inhibited TNFα-induced VCAM-1 expression at 1 and 9 dyn/cm2. b Ginkgolide B decreased TNFα-induced VE-cadherin expression at 1 and 9 dyn/cm2. c Ginkgolide B reduced TNFα-induced Cx43 expression at 1 and 9 dyn/cm2. #p < 0.05, significant difference between TNFα-treated and -untreated HUVECs. *p < 0.05, **p < 0.01, significant difference between TNFα-treated cells and ginkgolide B- and aspirin-treated cells

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