Table 1 PCR primer sequences
Primer Type | Primer name | Primer Sequence |
|---|---|---|
Mouse | IL-2 | F: 5’-GCA GCT GTT GAT GGA CCT AC-3′ |
R: 5’-TCC ACC ACA GTT GCT GAC TC-3′ | ||
IL-4 | F: 5’-TCG GCA TTT TGA ACG AGG TC-3′ | |
R: 5′-GAA AAG CCC GAA AGA GTC TC-3′ | ||
IL-13 | F: 5’-CGG CAG CAT GGT ATG GAG TG-3′ | |
R: 5′-ATT GCA ATT GGA GAT GTT GGT CAG-3′ | ||
iNOS | F: 5′-AAT GGC AAC ATC AGG TCG GCC ATC ACT-3′ | |
R: 5’-GCT GTG TGT CAC AGA AGT CTC GAA CTC-3′ | ||
TNF-α | F: 5′-ATG AGC ACA GAA AGC ATG ATC-3′ | |
R: 5’-TAC AGG CTT GTC ACT GGA ATT-3’ | ||
GAPDH | F: 5′-GAG GGG CCA TCC ACA GTC TTC-3’ | |
R: 5’-CAT CAC CAT CTT CCA GGA GCG-3’ | ||
Human | IL-4 | F: 5’-TGC CTC CAA GAA CAC AAC TG-3’ |
R: 5’-CTC TGG TTG GCT TCC TTC AC-3’ | ||
IL-6 | F: 5’-AAC CTT CCA AAG ATG GCT GAA-3’ | |
R: 5’-CAG GAA CTG GAT CAG GAC TTT-3’ | ||
IL-13 | F: 5′-GGT CAA CAT CAC CCA GAA CC-3’ | |
R: 5′-TTT ACA AAC TGG GCC ACC TC-3’ | ||
TSLP | F: 5′-TAT GAG TGG GAC CAA AAG TAC CG-3’ | |
R: 5′-GGG ATT GAA GGT TAG GCT CTG G-3’ | ||
GAPDH | F: 5’-CGT CTT CAC CAC CAT GGA GA-3’ | |
R: 5’-CGG CCA TCA CGC CAC AGT TT-3’ |