Fig. 5

Effects of DPI or NAC on CBNP-induced iNOS (a) and COX-2 (b) expression in A549 cells. c Effects of L-NAME, NS-398, or DPI on CBNP-induced ROS production in A549 cells. d Effects of L-NAME or NS-398 on CBNP-induced Nox2 activation in A549 cells. Cells were pretreated with DPI, NAC, L-NAME, or NS-398 1 h before addition of CBNPs (25 μg/mL) for 24 h. ROS production was measured using H₂DCF-DA staining and detected the DCF fluorescence by flow cytometer. Bars represent the mean ± S.E.M. from six independent experiments. ^###P < 0.001 vs. control group (without any treatment). ^**P < 0.01, ^***P < 0.001 vs. CBNPs (25 μg/mL) group